What this page covers

This page explains:

  • common hormone tests discussed in fertility
  • what each hormone is involved in
  • how results are interpreted in primary care
  • limitations of hormone testing
  • when further investigation or referral may be needed

Why hormone tests are discussed in fertility

Hormone tests may be considered when there are concerns about:

  • irregular or absent periods
  • ovulation patterns
  • cycle timing
  • symptoms of hormonal imbalance
  • planning for future pregnancy
  • conditions such as PCOS or early menopause

Tests are usually selected based on symptoms rather than done routinely.

Common fertility-related hormone tests

FSH plays a role in stimulating ovarian follicle development.

FSH is sometimes discussed in relation to ovarian function. Levels can vary significantly depending on the timing of the test and stage of the menstrual cycle. A single result does not give a complete picture.

LH triggers ovulation.

An altered LH to FSH ratio may be seen in conditions such as PCOS, but results must be interpreted alongside symptoms and cycle history.

Oestradiol is a form of oestrogen involved in cycle regulation and ovulation.

Levels fluctuate throughout the menstrual cycle, so timing of testing is important. Results are interpreted in relation to cycle phase.

Progesterone rises after ovulation.

Progesterone testing is sometimes used to assess whether ovulation has occurred. Timing of the test is critical, and interpretation depends on cycle length and regularity.

AMH reflects the number of follicles present in the ovaries and is often discussed as a marker of ovarian reserve.

Important points about AMH:

  • it does not predict natural fertility
  • it does not indicate egg quality
  • low levels do not mean pregnancy is impossible
  • high levels may be seen in PCOS

AMH is best interpreted as part of a broader assessment.

Raised prolactin levels can interfere with ovulation and menstrual cycles.

Prolactin may be tested when periods are absent, irregular or when there are symptoms such as nipple discharge.

Thyroid hormones play an important role in menstrual regularity and ovulation.

Both overactive and underactive thyroid conditions can affect fertility and cycle patterns.

Hormone tests have important limitations:

  • results fluctuate naturally
  • timing within the cycle matters
  • results must be interpreted together
  • blood tests do not assess egg quality or tubal factors
  • partner factors are not assessed by hormone tests

Testing should be guided by symptoms and clinical context rather than used as screening.


Further Reading and Hub Links

Visit our Women’s Health hub or browse more health topics in the AccessGP Knowledge Base.

If you would like to discuss menstrual cycles, hormone results or future family planning, you can book an online GP appointment with AccessGP.

Last reviewed by Dr Zamiel Hussain, GMC registered GP
Clinical contributor: Dr Tasnimah Miah, Women’s Health Advocate
Updated: 17 December 2025